The Repsol Honda rider crashed at Turn 1 around the halfway mark of the hour-long session, ending his day.
It marked the 2020 MotoGP world champion’s 12th crash so far this season, more than he had in the whole of 2022 while riding for Suzuki.
Honda said Mir had suffered damage around the fifth finger of the right hand, and would not continue with the weekend due to the “demanding nature of the Mugello Circuit.”
The team said: “Joan Mir had a difficult first day at the Italian GP, a crash mid-way through second practice ending his day early.
“Suffering significant discomfort, the #36 went to have his hand examined and revealed damage around the fifth finger of the right hand.
“Due to the high-speed and demanding nature of the Mugello Circuit, Joan Mir and the Repsol Honda Team have decided to withdraw from the remainder of the event due to the injury sustained to his right hand.”
Marc Marquez will now be the team’s sole factory rider for this weekend’s races, having returned from injury at the last round in France, where he was in podium contention before crashing.
Source: Autosport