The Australian, who currently competes in Formula 2, will drive Pierre Gasly’s A523 for the first practice session at Autodromo Hermanos Rodrigues in Mexico City next weekend.
He will then head out in Esteban Ocon’s car at the Abu Dhabi season finale next month.
Doohan also took part in two FP1 sessions last year for the team, at the same two circuits, and has undertaken extensive testing this season.
The 20-year-old is fourth in the F2 standings, driving for Virtuosi, and shook off a tough start to his campaign with back-to-back race wins in Hungary and Belgium as well as a further two podium appearances.
He finished sixth in the championship in his rookie season last year, with three wins and six podiums.
Doohan said: “It is a huge honour to be given the opportunity to drive a Formula 1 car on a grand prix weekend for the second year in a row.
“I am very grateful to BWT Alpine F1 Team for the chance to drive the A523 in Free Practice in both Mexico and Abu Dhabi.
“I have been working really hard with the team this year as their reserve driver, taking every opportunity to learn as much as possible for them and I feel prepared to step up and take over driving duties for the team.
Source: Autosport